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3921547

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Amplified expression of streptomyces endo-beta-N ...

3921547 [PubMed - indexed for MEDLINE] Publication Types: Research Support, U.S. Gov't, P.H.S. MeSH Terms. Acetylglucosaminidase/genetics* Acetylglucosaminidase/isolation & purification; Amino Acid Sequence; Chromatography, Gel; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics* Gene Amplification* Hexosaminidases/genetics*

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Amplified expression of streptomyces endo-beta-N ...

The endo-beta-N-acetylglucosaminidase H (Endo H) gene from Streptomyces plicatus has been cloned into the Escherichia coli plasmid pKC30 (Shimatake, H., and Rosenberg, M. (1981) Nature 272, 128-132), thus placing expression of this gene under control of the strong lambda promoter pL.

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